Welcome to Assay Calculator


This web application is designed to help you to analyse your data.
It can process data obtained from aequorin luminescence measurements ('Calcium Assay') or ROS measurements ('ROS Assay').

To view well plots and to start the calculations open the 'Data Analysis' tab.

Here are some things you have to keep in mind:

  • Input files have to be .xls, .xlsx or .csv files
  • Data has to be formatted accordingly, one column per well
  • The plate layout is optional, but it is required for the ROS normalization and for every mean, maximum and area calculation
  • ROS layout: put 'control' in the elicitor column for the measurements used for blanking
  • The measurement interval defaults to 10 s for the calcium assay and 60 s for the ROS assay and can be changed in the well curve settings; it scales the time axis and the calcium normalization
  • Every setting that influenced the numbers is written to the 'settings' sheet of the download

    • Download example data or empty templates:

  • Get

    Calcium Assay data

    and

    Calcium Assay plate layout

  • Get

    ROS Assay data

    and

    ROS Assay plate layout

  • Get empty

    data template

    and

    plate layout template

    • Assay Calculator was created by Alexander Kutschera (TU Munich) as an OpenPlantScience community project and is maintained in this fork by the Ranf lab.

    Please send your

    Assay Calculator is licenced with the

    GNU General Public License v3.0

    Well Curves

    Well Curves Settings

    Measurement

    Options

    Maxima of the Mean with SD

    Plot Settings

    Area under Curve of the Mean with SD

    Plot Settings

    Integration

    Mean Kinetics

    Plot Settings

    Combine raw files



    Download File

    Well Curves Settings

    Upload assay data with wildtype plants for comparison


    Mapping Well Curves

    Well Curves Settings

    Upload additional datasets for comparison


    Multiple Well Curves

    Download Everything!

    Download Data

    Download Plots